![]() In addition, IL-4 increases eotaxin expression through activation of Janus family tyrosine kinases and phosphorylation of signal transducer and activator of transcription (STAT), hence promotes eosinophilic inflammation by inducing eosinophil chemotaxis 4. It is associated with induction of Th2 differentiation as well as allergen specific IgE secretion by B cells 3. Among which interleukin (IL)−4 is a cytokine regulating development of allergic inflammation. In this study, we have demonstrated the in vitro and in vivo anti-asthma effects of CPPecp.Īsthma is a chronic, ongoing lung disease marked by acute flare-ups or attacks of difficulty with breathing such as shortness of breath, cough, chest tightness, and wheezing sound, and characterized by reversible obstruction of airway hyper-responsiveness (AHR) and airway inflammation in which eosinophil, lymphocytes, and neutrophil infiltrate airway 1, 2.ī cells, T-helper 2 (Th2) cells and Th2 related mediators play key roles in inflammatory responses in allergic asthma. CPPecp treated groups also showed lower serum mite-specific IgE level. In vivo studies showed CPPecp decreased mite-induced airway inflammation in terms of eosinophil and neutrophil count in broncho-alveolar lavage fluid, peri-bronchiolar and alveolar pathology scores, cytokine production in lung protein extract including interleukin (IL)-5, IL-13, IL-17A/F, eotaxin and pause enhancement from methacholine stimulation. CPPecp also decreases eotaxin secretion and p-STAT6 activation induced by ECP, as well as by IL-4. In BEAS-2B cell, CPPecp decreases ECP-induced eotaxin mRNA expression. Here we investigated airway-protective effects of CPPecp in BEAS-2B cell line and mite-induced airway allergic inflammation in BALB/c mice. ![]() It binds and penetrates bronchial epithelial cells without cytotoxic effects. ![]() Cell penetrating peptide derived from human eosinophil cationic protein (CPPecp) is a 10-amino-acid peptide containing a core heparan sulfate (HS)-binding motif of human eosinophil cationic protein (ECP). ![]()
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